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STEMCELL Technologies Inc rosetteseptm human nk cell enrichment cocktail
Rosetteseptm Human Nk Cell Enrichment Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosetteseptm+human+nk+cell+enrichment+cocktail/rosettesep+human+b+cell+enrichment+cocktail/pm40653772-207-8-14
Average 90 stars, based on 1 article reviews
rosetteseptm human nk cell enrichment cocktail - by Bioz Stars, 2026-09
90/100 stars

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Purification:

Article Title: CD44v6 specific CAR-NK cells for targeted immunotherapy of head and neck squamous cell carcinoma
Article Snippet: Regular testing for Mycoplasma was performed for all cell lines using a LookOut ® Mycoplasma PCR Detection Kit (Sigma-Aldrich, St. Louise, United States). .. Primary NK cells were purified from healthy donor buffy coats by negative selection using the RosetteSepTM human NK cell enrichment cocktail according to the manufacturer’s instructions (STEMCELL Technologies, Vancouver, Canada). .. After NK cell isolation, 1 × 10 6 cells per well were cultivated in a 24-well plate, in 1 ml of NK MACS ® medium (Miltenyi Biotec, Bergisch-Gladbach, Germany) supplemented with 5% (v/v) human serum (Sigma-Aldrich, St. Louise, United States), 500 IU/ml of recombinant rhIL-2 Proleukin ® S and 140 IU/ml of IL-15 (Miltenyi Biotec, Bergisch-Gladbach, Germany).

Selection:

Article Title: CD44v6 specific CAR-NK cells for targeted immunotherapy of head and neck squamous cell carcinoma
Article Snippet: Regular testing for Mycoplasma was performed for all cell lines using a LookOut ® Mycoplasma PCR Detection Kit (Sigma-Aldrich, St. Louise, United States). .. Primary NK cells were purified from healthy donor buffy coats by negative selection using the RosetteSepTM human NK cell enrichment cocktail according to the manufacturer’s instructions (STEMCELL Technologies, Vancouver, Canada). .. After NK cell isolation, 1 × 10 6 cells per well were cultivated in a 24-well plate, in 1 ml of NK MACS ® medium (Miltenyi Biotec, Bergisch-Gladbach, Germany) supplemented with 5% (v/v) human serum (Sigma-Aldrich, St. Louise, United States), 500 IU/ml of recombinant rhIL-2 Proleukin ® S and 140 IU/ml of IL-15 (Miltenyi Biotec, Bergisch-Gladbach, Germany).

Article Title: Comparative analysis of iPSC-derived NK cells from two differentiation strategies reveals distinct signatures and cytotoxic activities
Article Snippet: .. Primary NK cells were harvested by negative selection from healthy donor blood using RosetteSepTM Human NK Cell Enrichment Cocktail (Stemcell Tech, #15025). .. Total RNA was extracted using the RNeasy Mini Kit (Qiagen, #74104).

Isolation:

Article Title: Tuning antibody stability and function by rational designs of framework mutations.
Article Snippet: Optical absorbance was read on FLUOstar Omega spectrophotometer (BMG Labtech). .. Natural killer (NK) cells were isolated using the RosetteSepTM Human NK Cell Enrichment Cocktail (STEMCELL Technologies) following the manufacturer’s instructions. ..

Article Title: Optimized peptide nanofibrils as efficient transduction enhancers for in vitro and ex vivo gene transfer
Article Snippet: .. NK cells were isolated from buffy coats of healthy donors by standard density-gradient centrifugation, using Ficoll-Paque (VWR, Cat: 17-1440-03) using the RosetteSepTM Human NK Cell Enrichment Cocktail (STEMCELL Technologies, Cat: 15065). .. NK cells were cultured at 1×10 6 cells/ml in NK MACS medium (Miltenyi Biotec, Cat: 130-114-429) with 5% human AB serum (Sigma Aldrich, Cat: H4522-100ML), 500 U/ml IL-2 (Peprotech, Cat: 200-02) and 140 U/ml IL-15 (Peprotech, Cat: 200-15).

Article Title: Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells
Article Snippet: .. Cells were then isolated by standard density-gradient centrifugation using Ficoll-Paque (VWR, Germany, Catalog. Nr. 17-1440-03) using the RosetteSepTM Human NK Cell Enrichment Cocktail (Stemcell Technologies, Canada) for NK cells, the RosetteSepTM Human B Cell Enrichment Cocktail for B cells and the RosetteSepTM Human Monocyte Enrichment Cocktail for monocytes, respectively. .. NK cells were cultured at 1 × 10 6 cells/mL in NK MACS medium (Miltenyi Biotec, Germany) with 5% human AB serum (Sigma Aldrich, USA, Catalog Nr. H4522-100ML), 500 U/mL IL-2 and 140 U/mL IL-15 (Peprotech, USA).

Article Title: Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells.
Article Snippet: .. Cells were then isolated by standard density- gradient centrifugation using Ficoll-Paque (VWR, Germany, Catalog. Nr. 17- 1440-03) using the RosetteSepTM Human NK Cell Enrichment Cocktail (Stemcell Technologies, Canada) for NK cells, the RosetteSepTM Human B Cell Enrichment Cocktail for B cells and the RosetteSepTM Human Monocyte Enrichment Cocktail for monocytes, respectively. .. NK cells were cultured at 1 × 106 cells/mL in NK MACS medium (Miltenyi Biotec, Germany) with 5% human AB serum (Sigma Aldrich, USA, Catalog Nr. H4522-100ML), 500 U/mL IL-2 and 140 U/mL IL-15 (Peprotech, USA).

Article Title: Derivation and Preclinical Characterization of CYT-303, a Novel NKp46-NK Cell Engager Targeting GPC3.
Article Snippet: .. PBNKs were isolated from healthy blood donors by negative selection using the RosetteSepTM Human NK Cell Enrichment Cocktail (Stemcell Technologies, Cambridge, MA, USA). ..

other:

Article Title: Allogeneic CAR-T cells with of HLA-A/B and TRAC disruption exhibit promising antitumor capacity against B cell malignancies.
Article Snippet: Human primary NK cell isolation and culture Human primary NK cells were isolated using a negative selection kit (RosetteSepTM Human NK Cell Enrichment Cocktail; Stem Cell Technologies, Vancouver, Canada).

Centrifugation:

Article Title: Optimized peptide nanofibrils as efficient transduction enhancers for in vitro and ex vivo gene transfer
Article Snippet: .. NK cells were isolated from buffy coats of healthy donors by standard density-gradient centrifugation, using Ficoll-Paque (VWR, Cat: 17-1440-03) using the RosetteSepTM Human NK Cell Enrichment Cocktail (STEMCELL Technologies, Cat: 15065). .. NK cells were cultured at 1×10 6 cells/ml in NK MACS medium (Miltenyi Biotec, Cat: 130-114-429) with 5% human AB serum (Sigma Aldrich, Cat: H4522-100ML), 500 U/ml IL-2 (Peprotech, Cat: 200-02) and 140 U/ml IL-15 (Peprotech, Cat: 200-15).

Article Title: Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells
Article Snippet: .. Cells were then isolated by standard density-gradient centrifugation using Ficoll-Paque (VWR, Germany, Catalog. Nr. 17-1440-03) using the RosetteSepTM Human NK Cell Enrichment Cocktail (Stemcell Technologies, Canada) for NK cells, the RosetteSepTM Human B Cell Enrichment Cocktail for B cells and the RosetteSepTM Human Monocyte Enrichment Cocktail for monocytes, respectively. .. NK cells were cultured at 1 × 10 6 cells/mL in NK MACS medium (Miltenyi Biotec, Germany) with 5% human AB serum (Sigma Aldrich, USA, Catalog Nr. H4522-100ML), 500 U/mL IL-2 and 140 U/mL IL-15 (Peprotech, USA).

Gradient Centrifugation:

Article Title: Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells.
Article Snippet: .. Cells were then isolated by standard density- gradient centrifugation using Ficoll-Paque (VWR, Germany, Catalog. Nr. 17- 1440-03) using the RosetteSepTM Human NK Cell Enrichment Cocktail (Stemcell Technologies, Canada) for NK cells, the RosetteSepTM Human B Cell Enrichment Cocktail for B cells and the RosetteSepTM Human Monocyte Enrichment Cocktail for monocytes, respectively. .. NK cells were cultured at 1 × 106 cells/mL in NK MACS medium (Miltenyi Biotec, Germany) with 5% human AB serum (Sigma Aldrich, USA, Catalog Nr. H4522-100ML), 500 U/mL IL-2 and 140 U/mL IL-15 (Peprotech, USA).



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